pCT5-bac1.8
(Plasmid #119871)

• Purpose: Constitutive expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coli
• Depositing Lab: Claudia Schmidt-dannert
• Publication: Seo et al Appl Microbiol Biotechnol. 2018 Nov 3. pii: 10.1007/s00253-018-9485-4. doi: 10.1007/s00253-018-9485-4.

Backbone

• Vector backbone: pRBBm34
• Backbone manufacturer: Biedendieck et al., 2007
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: For Bacillus: Tetracycline and 30 °C or 37 °C
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: CymR'
• Species: Pseudomonas putida F1
• Insert Size (bp): 624
• Mutation: SNP in 592 bp
• Promoter: PserA promoter

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: taacctttgcgttcacttaaactagtTCACACTGGCTCACCTTC

Gene/Insert 2

• Gene/Insert name: sfGFP
• Species: Synthetic; superfolding GFP
• Insert Size (bp): 717
• Promoter: Pveg promoter with CuO sequence

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: acgattcggaTCCTGTATTACTATTCTATTTTGTC

Resource Information

• Supplemental Documents:
  • pCT5-bac1.8.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry