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pGEM-BirA-2A-H2B-Dendra2-SV40pA-FKF
(Plasmid #119864)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 119864 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEM-T
  • Backbone size w/o insert (bp) 3017
  • Total vector size (bp) 7063
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BirA, 2A, H2B-tagged Dendra2 and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    4046
  • Tags / Fusion Proteins
    • histone 2B (N terminal on insert)
    • HA-tagged biotin ligase (BirA), Thosea asigna virus peptide (2A) (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEM-BirA-2A-H2B-Dendra2-SV40pA-FKF was a gift from Tatjana Sauka-Spengler (Addgene plasmid # 119864 ; http://n2t.net/addgene:119864 ; RRID:Addgene_119864)

  • For your References section:

    Functional Heterogeneity within the Developing Zebrafish Epicardium. Weinberger M, Simoes FC, Patient R, Sauka-Spengler T, Riley PR. Dev Cell. 2020 Mar 9;52(5):574-590.e6. doi: 10.1016/j.devcel.2020.01.023. Epub 2020 Feb 20. 10.1016/j.devcel.2020.01.023 PubMed 32084358
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