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pAL725
(Plasmid #119862)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 119862 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQE-2
  • Backbone manufacturer
    QIAgen
  • Backbone size w/o insert (bp) 4758
  • Total vector size (bp) 5676
  • Modifications to backbone
    remove Histidine tag
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    MM52
  • Growth instructions
    lambda DE3 lysogen not required Change media and shift temperature to 39 degrees with IPTG added to produce the precursor proteins
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    D-galactose/methyl-galactoside ABC transporter periplasmic binding protein, mglB, L244C, D287C
  • Alt name
    ECK2143
  • Species
    Escherichia coli str. K-12
  • Mutation
    Leucine 244 to Cysteine, Aspartate 287 to Cysteine
  • GenBank ID
    949041
  • Entrez Gene
    mglB (a.k.a. b2150, ECK2143)
  • Promoter T5
  • Tag / Fusion Protein
    • none

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer 5' - GCGGCGAATCAGGGTGG - 3'
  • 3′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Vector contains T5 promoter, lac operator, laclq repressor gene and Col E1 origin of replication. The original 6xHis tag sequence of pQE-2 has been removed from this construct.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAL725 was a gift from Linda Randall (Addgene plasmid # 119862 ; http://n2t.net/addgene:119862 ; RRID:Addgene_119862)

  • For your References section:

    Co-assembly of SecYEG and SecA fully restores the properties of the native translocon. Bariya P, Randall LL. J Bacteriol. 2018 Oct 1. pii: JB.00493-18. doi: 10.1128/JB.00493-18. 10.1128/JB.00493-18 PubMed 30275279
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