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Addgene

Cmv d0 dPspCas13b-GGS-NYTHDF2(100-200)
(Plasmid #119856)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 119856 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    cmv d0
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dCas13b-GGS-NYTHDF2(100-200)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer AGTTAAATTGCTAACGCAGTCA
  • 3′ sequencing primer CAGCAGCCAACTCAGCTTCCTTTCGGGCTT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Cmv d0 dPspCas13b-GGS-NYTHDF2(100-200) was a gift from Bryan Dickinson (Addgene plasmid # 119856 ; http://n2t.net/addgene:119856 ; RRID:Addgene_119856)

  • For your References section:

    Targeted m(6)A Reader Proteins To Study Epitranscriptomic Regulation of Single RNAs. Rauch S, He C, Dickinson BC. J Am Chem Soc. 2018 Sep 26;140(38):11974-11981. doi: 10.1021/jacs.8b05012. Epub 2018 Sep 18. 10.1021/jacs.8b05012 PubMed 30183280
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