pAL928
(Plasmid #119853)

• Purpose: precusor alkaline phosphatase, phoA, with C169S, C179S, C287S, C337S, A369C and A427C
• Depositing Lab: Linda Randall
• Publication: Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-18. doi: 10.1128/JB.00493-18.

Backbone

• Vector backbone: pBR322 with T7 promoter
• Total vector size (bp): 2788
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): BL21.19
• Growth instructions: Change media and shift temperature to 39 degrees with IPTG added to produce the precursor proteins
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: precursor alkaline phosphatase, phoA, with mutations C169S, C179S, C287S, C337S, A369C and A427C
• Alt name: ECK0378; psiA
• Species: Escherichia coli (strain K12)
• Mutation: Cysteine 169 to Serine, Cysteine 179 to Serine, Cysteine 287 to Serine, Cysteine 337 to Serine, Alanine 369 to Cysteine and Alanine 427 to Cysteine
• GenBank ID: 945041
• Promoter: T7
• Tag / Fusion Protein:
  • none

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: 5' CTGGGCGTCGATATTCAC 3', 5' CACAGGCGCGTGGTTATC 3', 5' ACGCCAGCCAGATTGTTG 3'
• 3′ sequencing primer: 5' GCCCGTGATCTGCCATTA 3'

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Donald Oliver at Wesleyan University

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at or contact our distributors if you have any questions.