pET41a iGluu
(Plasmid #119834)

• Purpose: Production of recombinant iGluSnFR S72T ultrafast variant, a single-wavelength glutamate indicator, in E. coli
• Depositing Lab: Katalin Torok
• Publication: Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pii: 1720648115. doi: 10.1073/pnas.1720648115.

Backbone

• Vector backbone: pET41a
• Backbone manufacturer: EMD Biosciences
• Backbone size w/o insert (bp): 5933
• Total vector size (bp): 7496
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: iGluu
• Alt name: iGluSnFR S72T
• Species: Synthetic; E. coli
• Insert Size (bp): 1563
• Mutation: S72T
• Promoter: T7
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7 fwd

Resource Information

• Supplemental Documents:
  • pET41a-iGluSnFR S72T.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry