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Addgene

pET41a iGluSnFR E25R
(Plasmid #119831)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 119831 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET41a
  • Backbone manufacturer
    EMD Biosciences
  • Backbone size w/o insert (bp) 5933
  • Total vector size (bp) 7496
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    iGluSnFR E25R
  • Species
    Synthetic; E. coli
  • Insert Size (bp)
    1563
  • Mutation
    E25R
  • Promoter T7
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7 fwd
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET41a iGluSnFR E25R was a gift from Katalin Torok (Addgene plasmid # 119831 ; http://n2t.net/addgene:119831 ; RRID:Addgene_119831)

  • For your References section:

    Ultrafast glutamate sensors resolve high-frequency release at Schaffer collateral synapses. Helassa N, Durst CD, Coates C, Kerruth S, Arif U, Schulze C, Wiegert JS, Geeves M, Oertner TG, Torok K. Proc Natl Acad Sci U S A. 2018 May 7. pii: 1720648115. doi: 10.1073/pnas.1720648115. 10.1073/pnas.1720648115 PubMed 29735711
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