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PurposeFor cloning and subsequent expression of peptide fragments fused with Avi-tag (for biotinylation) and with pIII protein of M13K07 bacteriophage
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 119819 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHen2
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Modifications to backboneNcoI/NheI restriction sites for subsequent peptide cloning
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsThe TG1 strain should be used for bacteriophage infection and subsequent expression
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namelinker with Avi-tag and p3
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Tag
/ Fusion Protein
- Avi-tag (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHen2 Avi-tag-linker-p3 was a gift from Alexander Gabibov (Addgene plasmid # 119819 ; http://n2t.net/addgene:119819 ; RRID:Addgene_119819) -
For your References section:
Probing surface membrane receptors using engineered bacteriophage bioconjugates. Lomakin YA, Kaminskaya AN, Stepanov AV, Shmidt AA, Gabibov A, Belogurov AA. Bioconjug Chem. 2019 Apr 25. doi: 10.1021/acs.bioconjchem.9b00218. 10.1021/acs.bioconjchem.9b00218 PubMed 31021608