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Addgene

BirA His-tag pLEMO
(Plasmid #119817)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 119817 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLEMO
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    rhamnose promoter
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Biotin ligase
  • Alt name
    BirA
  • Insert Size (bp)
    993
  • Tag / Fusion Protein
    • 6-Histidine-tag (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BirA His-tag pLEMO was a gift from Alexander Gabibov (Addgene plasmid # 119817 ; http://n2t.net/addgene:119817 ; RRID:Addgene_119817)

  • For your References section:

    Probing surface membrane receptors using engineered bacteriophage bioconjugates. Lomakin YA, Kaminskaya AN, Stepanov AV, Shmidt AA, Gabibov A, Belogurov AA. Bioconjug Chem. 2019 Apr 25. doi: 10.1021/acs.bioconjchem.9b00218. 10.1021/acs.bioconjchem.9b00218 PubMed 31021608
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