pLentipuro3/TO/V5-GW/EGFP-Firefly Luciferase
(Plasmid #119816)

• Purpose: 3rd generation lentiviral plasmid for expression of EGFP Firefly Luciferase fusion protein in mammalian cells
• Depositing Lab: Ethan Abel
• Publication: Lorenzatti Hiles et al Nat Protoc. 2019 Mar;14(3):738-755. doi: 10.1038/s41596-018-0112-8.

Backbone

• Vector backbone: pLentipuro3/TO/V5-DEST
• Backbone size w/o insert (bp): 8116
• Total vector size (bp): 10489
• Vector type: Mammalian Expression, Lentiviral, Luciferase
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Luciferase
• Alt name: Firefly luciferase
• Species: Photinus pyralis
• Insert Size (bp): 2373
• Mutation: Stop codon removed in the frame of the V5 frame vector
• Promoter: CMV/TO
• Tags / Fusion Proteins:
  • EGFP (N terminal on insert)
  • V5 (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: V5 Reverse

Gene/Insert 2

• Gene/Insert name: Puromycin
• Species: Streptomyces alboniger
• Insert Size (bp): 600
• Promoter: Human Phosphoglycerate kinase

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: Unknown
• 3′ sequencing primer: Unknown

Resource Information

• Supplemental Documents:
  • Seq_pLentipuro3TOV5-GWEGFP-Firefly Luciferase.gb
• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry