KFP14
(Plasmid
#119796)
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PurposeRpoD6 epitope tagged construct for integration at neutral site 1
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 119796 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneVector for targeting integration at neutral site 1 in ATCC PCC7942 genome
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin and Streptomycin, 50 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSynpcc7942_1557
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Entrez GeneSynpcc7942_1557 (a.k.a. Synpcc7942_1557)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
KFP14 was a gift from Erin O'Shea (Addgene plasmid # 119796 ; http://n2t.net/addgene:119796 ; RRID:Addgene_119796) -
For your References section:
An RpaA-Dependent Sigma Factor Cascade Sets the Timing of Circadian Transcriptional Rhythms in Synechococcus elongatus. Fleming KE, O'Shea EK. Cell Rep. 2018 Dec 11;25(11):2937-2945.e3. doi: 10.1016/j.celrep.2018.11.049. 10.1016/j.celrep.2018.11.049 PubMed 30540929