pET-mGinger1
(Plasmid
#119784)
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PurposeExpresses mGinger1 tagged N-terminally with an MHHHHHHGSG tag
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 119784 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET-53-DEST
- Backbone size w/o insert (bp) 5153
- Total vector size (bp) 5810
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemGinger1
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SpeciesSynthetic
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Insert Size (bp)657
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GenBank IDMK040729
- Promoter T7
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Tag
/ Fusion Protein
- 6xHis tag (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET-mGinger1 was a gift from Stephen Mayo (Addgene plasmid # 119784 ; http://n2t.net/addgene:119784 ; RRID:Addgene_119784) -
For your References section:
Monomerization of far-red fluorescent proteins. Wannier TM, Gillespie SK, Hutchins N, McIsaac RS, Wu SY, Shen Y, Campbell RE, Brown KS, Mayo SL. Proc Natl Acad Sci U S A. 2018 Nov 27;115(48):E11294-E11301. doi: 10.1073/pnas.1807449115. Epub 2018 Nov 13. 10.1073/pnas.1807449115 PubMed 30425172
