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Addgene

pET-mKelly2
(Plasmid #119783)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 119783 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-53-DEST
  • Backbone size w/o insert (bp) 5153
  • Total vector size (bp) 5810
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mKelly2
  • Species
    Synthetic
  • Insert Size (bp)
    657
  • GenBank ID
    MK040732
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis tag (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-mKelly2 was a gift from Stephen Mayo (Addgene plasmid # 119783 ; http://n2t.net/addgene:119783 ; RRID:Addgene_119783)

  • For your References section:

    Monomerization of far-red fluorescent proteins. Wannier TM, Gillespie SK, Hutchins N, McIsaac RS, Wu SY, Shen Y, Campbell RE, Brown KS, Mayo SL. Proc Natl Acad Sci U S A. 2018 Nov 27;115(48):E11294-E11301. doi: 10.1073/pnas.1807449115. Epub 2018 Nov 13. 10.1073/pnas.1807449115 PubMed 30425172
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