p3xFLAG-MKL1
(Plasmid #11978)

• Purpose: For mammalian expression of human MKL1 with a 3x FLAG tag
• Depositing Lab: Ron Prywes
• Publication: Cen et al Mol Cell Biol. 2003 Sep . 23(18):6597-608.

Backbone

• Vector backbone: pCMV-3xFLAG-7.1
• Backbone manufacturer: Sigma
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MKL1
• Species: H. sapiens (human)
• Insert Size (bp): 2800
• Mutation: N579S T716A
• Entrez Gene: MRTFA (a.k.a. BSAC, MAL, MKL, MKL1, MRTF-A)
• Promoter: CMV
• Tag / Fusion Protein:
  • 3xFLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: hGHpA-R

Resource Information

• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry