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pET42b-A3A-PBE-ΔUGI
(Plasmid #119771)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 119771 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET42b
  • Backbone size w/o insert (bp) 4900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    For protein expression, use BL21 star cells.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    APOBEC3A-nCas9-NLS
  • Alt name
    APOBEC3A-nCas9-ΔUGI
  • Insert Size (bp)
    4830
  • Mutation
    nCas9 has a mutation D10A in wild type SpCas9
  • Promoter T7 promoter
  • Tags / Fusion Proteins
    • 6*HIS (N terminal on insert)
    • NLS (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET42b-A3A-PBE-ΔUGI was a gift from Caixia Gao (Addgene plasmid # 119771 ; http://n2t.net/addgene:119771 ; RRID:Addgene_119771)

  • For your References section:

    Efficient C-to-T base editing in plants using a fusion of nCas9 and human APOBEC3A. Zong Y, Song Q, Li C, Jin S, Zhang D, Wang Y, Qiu JL, Gao C. Nat Biotechnol. 2018 Oct 1. pii: nbt.4261. doi: 10.1038/nbt.4261. 10.1038/nbt.4261 PubMed 30272679
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