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pRN3P_TIR1
(Plasmid #119766)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 119766 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRN3P

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    TIR1

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note that the sequence contains some SNPs compared to the reference Os genome. However, TIR expression from this plasmids works well in controlling auxin-inducible degron in mouse cells in our hands

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRN3P_TIR1 was a gift from Maria-Elena Torres-Padilla (Addgene plasmid # 119766 ; http://n2t.net/addgene:119766 ; RRID:Addgene_119766)

  • For your References section:

    Genome-lamina interactions are established de novo in the early mouse embryo. Borsos M, Perricone SM, Schauer T, Pontabry J, de Luca KL, de Vries SS, Ruiz-Morales ER, Torres-Padilla ME, Kind J. Nature. 2019 May;569(7758):729-733. doi: 10.1038/s41586-019-1233-0. Epub 2019 May 22. 10.1038/s41586-019-1233-0 PubMed 31118510
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