p5xATF6-GL3
(Plasmid #11976)

• Purpose: Reporter gene with five ATF6 sites that is highly sensitive to ER stress
• Depositing Lab: Ron Prywes
• Publication: Wang et al J Biol Chem. 2000 Sep 1. 275(35):27013-20.

Backbone

• Vector backbone: pOFluc-GL3
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 5xATF6 binding site
• Tags / Fusion Proteins:
  • Luc (C terminal on backbone)
  • c-fos promoter (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: NA

Resource Information

• Articles Citing this Plasmid:
  • 36 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

5 repeats of the ATF6 oligonucleotides were cloned into pOFluc-GL3 at an XhoI site 5' to the c-fos minimal promoter (-53 to +45 of the human c-fos promoter) and the firefly luciferase gene. See author's map for detail.

Oligo seq is - 5'- TCGAGACAGGTGCTGACGTGGCGATTCC