pGEX6P1-N-His
(Plasmid #119755)

• Purpose: (Empty Backbone) Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal 10x His tag.
• Depositing Labs: Andrew Jackson, Martin Reijns
• Publication: Bacterial expression plasmids for production of recombinant proteins with a PreScission Protease cleavable GST tag and additional small affinity tags (unpublished)

Backbone

• Vector backbone: pGEX-6-P1
• Backbone manufacturer: Amersham
• Backbone size (bp): 5000
• Modifications to backbone: 10xHis tag cloned into BamHI site of pGEX6P1, BamHI site only intact on 5' end
• Vector type: Bacterial Expression
• Promoter: tac promoter
• Tags / Fusion Proteins:
  • GST (N terminal on insert)
  • 10xHis (N terminal on insert)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: PGEX5' - GGGCTGGCAAGCCACGTTTGGTG
• 3′ sequencing primer: PGEX3' - CCGGGAGCTGCATGTGTCAGAGG

Resource Information

• Supplemental Documents:
  • pGEX6P1-N-His.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry