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Addgene

pCAG PSAM4-5HT3 HC IRES EGFP Notes

Addgene Diagnostic Digest-NheI/SacI

This gel depicts the NEB 1kb ladder and a digestion of the plasmid pCAG PSAM4-5HT3 HC IRES EGFP with the following enzymes: NheI-HF and SacI-HF.

We observe the expected results below:
NheI-HF : expect 1 band: 8664bp
NheI-HF/ SacI-HF: expect 2 bands: 5102 and 3562bp