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PurposeFor mammalian expression of human ATF6 with an HA tag
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 11974 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCGN
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameATF6
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SpeciesH. sapiens (human)
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Entrez GeneATF6 (a.k.a. ACHM7, ATF6A, ATP6alpha)
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Tag
/ Fusion Protein
- HA (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer NA (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
See author's map and paper for cloning details. pCGN: expression is driven by CMV promoter
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCGN-ATF6 was a gift from Ron Prywes (Addgene plasmid # 11974 ; http://n2t.net/addgene:11974 ; RRID:Addgene_11974) -
For your References section:
Interaction of ATF6 and serum response factor. Zhu C, Johansen FE, Prywes R. Mol Cell Biol. 1997 Sep . 17(9):4957-66. PubMed 9271374
Map uploaded by the depositor.