SynTagMA_pre
(Plasmid #119738)

• Purpose: Synaptic Tag for Mapping Activity
• Depositing Lab: Thomas Oertner
• Publication: Perez-Alvarez et al Nat Commun. 2020 May 18;11(1):2464. doi: 10.1038/s41467-020-16315-4.

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Backbone

• Vector backbone: pAAV
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 4597
• Total vector size (bp): 6874
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SynTagMA_pre
• Species: Synthetic
• Insert Size (bp): 2277
• Promoter: hsyn1

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: BsrGI (not destroyed)
• 5′ sequencing primer: syn-sense2 GACTCAGCGCTGCCTCAGTCTG
• 3′ sequencing primer: S-DIO-R GTTGATTATCGATAAGCTTG

Resource Information

• Supplemental Documents:
  • SyntagMa_pre.gb
• A portion of this plasmid was derived from a plasmid made by: Synaptophysin-CamPARI 2 was a kind gift from Mike Hoppa, Dartmouth College
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/538041v3 for BioRxiv preprint.