39R861+
(Bacterial strain #119737)

• Purpose: Six stable plasmids can be used as size standards
• Depositing Lab: Ruth Hall
• Publication: Moran et al Plasmid. 2019 Mar;102:6-9. doi: 10.1016/j.plasmid.2019.01.002. Epub 2019 Feb 7.

Backbone

• Vector backbone: See supplemental files for plasmid details

Growth in Bacteria

• Bacterial Resistance(s): None
• Growth Temperature: 37°C
• Growth Strain(s): E. coli K12
• Growth instructions: See comments below for additional information
• Copy number: Unknown

Resource Information

• Supplemental Documents:
  • Plasmid descriptions
  • Plasmid info with references
  • Gel Image of Plasmids

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Strain can be grown on LB without antibiotics.

Resistant to: ampicillin, chloramphenicol, florfenicol, gentamicin, kanamycin, nalidixic acid, streptomycin, spectinomycin, sulphonamides, tobramycin, tetracycline, trimethoprim

Please note that plasmids are stable in the absence of antibiotic selection.

39R861+ is resistant to nalidixic acid due to a chromosomal mutation. The remaining resistance determinants are plasmid-borne.

39R861+ contains six plasmids, outlined in the supplemental files.