Myc-gamma2/deltaILTM4
(Plasmid #119735)

• Purpose: GABAA receptor expression (chimeric rat rat gamma 2 subunit whose large intracellular loop, TM4 and C-term have been replaced with the equivalent domains of the rat delta subunit) C-myc near N-term
• Depositing Lab: Angel de Blas
• Publication: Wu et al J Biol Chem. 2012 Aug 10;287(33):27417-30. doi: 10.1074/jbc.M112.360461. Epub 2012 Jun 18.

Backbone

• Vector backbone: pCDNA3.1
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GABRG2/GABRD
• Species: R. norvegicus (rat)
• Entrez Gene: Gabrg2
• Entrez Gene: Gabrd (a.k.a. GABAA-RD)
• Tag / Fusion Protein:
  • cMyc epitope EQKLISEEDL inserted between fourth and fifth amino acids of mature protein after signal peptide is cleaved (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: unknown

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The gamma 2 gene was originally cloned in Peter Seeburg’s lab in Heidelberg. The rat delta was originally from Dr. Robert Macdonald at Vanderbilt.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry