Myc-delta/gamma2SIL
(Plasmid #119731)

• Purpose: GABAA receptor expression (chimeric rat delta subunit whose large intracellular loop has been replaced with the large intracellular loop of the rat gamma 2 short subunit) Myc-tag near N-terminus
• Depositing Lab: Angel de Blas
• Publication: de Blas Lab plasmids (unpublished)

Backbone

• Vector backbone: pCDNA3.1
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GABRD/GABRG2
• Species: R. norvegicus (rat)
• Entrez Gene: Gabrg2
• Entrez Gene: Gabrd (a.k.a. GABAA-RD)
• Tag / Fusion Protein:
  • cMyc epitope EQKLISEEDL inserted between fourth and fifth amino acids of mature protein after signal peptide is cleaved (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: unknown

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The gamma 2 gene was originally cloned in Peter Seeburg’s lab in Heidelberg. The rat delta was originally from Dr. Robert Macdonald at Vanderbilt.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry