Skip to main content
Addgene

mOrange-imp α
(Plasmid #119720)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 119720 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pmOrange-C1
  • Backbone manufacturer
    not commercially available one. Note that Eco47III, AgeI, BspE1 are missing
  • Modifications to backbone
    NheI-BglII DNA fragment encoding EGFP of pEGFP is replaced by the DNA fragment encoding mOrange, which is amplified by PCR using pRSET B mOrange (provided by Dr. Roger Y. Tsien).
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    importinα
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1000
  • Mutation
    contains amino acids 251-529
  • Entrez Gene
    KPNA2 (a.k.a. IPOA1, PTAC58, QIP2, RCH1, SRP1-alpha, SRP1alpha)
  • Promoter CMV
  • Tag / Fusion Protein
    • mOrange (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pmOrange-impα was constructed by replacing the AgeI-BglII fragment encoding DsRed of pDsRed-imp α (Addgene plasmid 119679) with mOrange derived from pRSET-B-mOrange (provided by Professor Roger Y. Tsien, UCSD).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mOrange-imp α was a gift from Kenji Sugimoto (Addgene plasmid # 119720 ; http://n2t.net/addgene:119720 ; RRID:Addgene_119720)

  • For your References section:

    A rapid and simple method of evaluating the dimeric tendency of fluorescent proteins in living cells using a truncated protein of importin alpha as fusion tag. Nakagawa C, Nishimura S, Senda-Murata K, Sugimoto K. Biosci Biotechnol Biochem. 2012;76(2):388-90. doi: 10.1271/bbb.110677. Epub 2012 Feb 7. 10.1271/bbb.110677 PubMed 22313767
Related items: