pEGFP-imp α
(Plasmid #119718)

• Purpose: Expresses EGFP-tagged human importinα in mammalian cells
• Depositing Lab: Kenji Sugimoto
• Publication: Nakagawa et al Biosci Biotechnol Biochem. 2012;76(2):388-90. doi: 10.1271/bbb.110677. Epub 2012 Feb 7.

Backbone

• Vector backbone: pEGFP-ΔC2
• Modifications to backbone: pEGFP-delat C2 is constructed by ligation of blunt-ended HindIII site of pEGFP-C1, resulting in the C2-type reading frame
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: importinα
• Species: H. sapiens (human)
• Mutation: contains amino acids 251-529
• Entrez Gene: KPNA2 (a.k.a. IPOA1, PTAC58, QIP2, RCH1, SRP1-alpha, SRP1alpha)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: SalI (unknown if destroyed)
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

1-kb fragment of the EcoRI-XhoI fragment of DsRed-imp alpha (Addgene plasmid 119679) was cloned into the EcoRI-SalI sites of pEGFP-ΔC2, a frame-shifted derivative of pEGFP-C1.