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PurposePlasmid to generate zGrad mRNA to inactivate proteins in zebrafish
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Depositing Lab
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Sequence Information
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Sequences (2) — Accept Affinity Reagent Sequence Policy
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Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 119716 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCS2
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namezGrad
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Alt namefusion of F-box from fbxw11b and anti-GFP lama single domain antibody vhhGFP4
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)1005
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer SP6
- 3′ sequencing primer T3 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2(+)-zGrad was a gift from Holger Knaut (Addgene plasmid # 119716 ; http://n2t.net/addgene:119716 ; RRID:Addgene_119716) -
For your References section:
zGrad is a nanobody-based degron system that inactivates proteins in zebrafish. Yamaguchi N, Colak-Champollion T, Knaut H. Elife. 2019 Feb 8;8. pii: 43125. doi: 10.7554/eLife.43125. 10.7554/eLife.43125 PubMed 30735119
