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Addgene

pCMVTAG-NEMOL329P
(Plasmid #11971)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11971 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMVTAG2B
  • Backbone size w/o insert (bp) 4300
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NEMOL329P
  • Alt name
    NEMO
  • Alt name
    IKKgamma
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1290
  • Mutation
    Leucine329 to Proline
  • GenBank ID
    NM_003639
  • Entrez Gene
    IKBKG (a.k.a. AMCBX1, EDAID1, FIP-3, FIP3, Fip3p, IKK-gamma, IKKAP1, IKKG, IMD33, IP, IP1, IP2, IPD2, NEMO, SAIDX, ZC2HC9)
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T3
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that Addgene's quality control sequence confirmed 2 additional amino acids changes in the NEMO ORF - A211T and L301M.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMVTAG-NEMOL329P was a gift from Jon Ashwell (Addgene plasmid # 11971 ; http://n2t.net/addgene:11971 ; RRID:Addgene_11971)

  • For your References section:

    NEMO is a sensor of Lys 63-linked polyubiquitination and functions in NF-kappaB activation. Wu CJ, Conze DB, Li T, Srinivasula SM, Ashwell JD. Nat Cell Biol. 2006 Apr . 8(4):398-406. 10.1038/ncb1384 PubMed 16547522
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