pAL829
(Plasmid #119697)

• Purpose: precursor slow-folding maltodextrin binding protein, MBP or malE, with mutations Y283D, G289C and V347C
• Depositing Lab: Linda Randall
• Publication: Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-18. doi: 10.1128/JB.00493-18.

Backbone

• Vector backbone: pQE-2
• Backbone manufacturer: QIAgen
• Total vector size (bp): 5868
• Modifications to backbone: remove Histidine tag
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): BL21.19
• Growth instructions: Change media and shift temperature to 39 degrees with IPTG added to produce the precursor proteins Does not require lambda DE3 lysogen
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: slow-folding maltose ABC transporter periplasmic binding protein, malE, with mutations Y283D, G289C and V347C
• Alt name: ECK4026, b4034, JW3994[1], JW3994, malJ
• Species: Escherichia coli (strain K12)
• Insert Size (bp): 1191
• Mutation: mutations Y283D, G289C and V347C
• GenBank ID: 948538
• Promoter: T5
• Tag / Fusion Protein:
  • none

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: 5' - GCC CAG AAA GGT GAA ATC - 3'
• 3′ sequencing primer: 5' - AAGGCGAAACAGCGATGA - 3'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Vector contains T5 promoter, lac operator, laclq repressor gene and Col E1 origin of replication. The original 6xHis tag sequence of pQE-2 has been removed from this construct.

Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at or contact our distributors if you have any questions.