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Addgene

pAL829
(Plasmid #119697)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 119697 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pQE-2
  • Backbone manufacturer
    QIAgen
  • Total vector size (bp) 5868
  • Modifications to backbone
    remove Histidine tag
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    BL21.19
  • Growth instructions
    Change media and shift temperature to 39 degrees with IPTG added to produce the precursor proteins Does not require lambda DE3 lysogen
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    slow-folding maltose ABC transporter periplasmic binding protein, malE, with mutations Y283D, G289C and V347C
  • Alt name
    ECK4026, b4034, JW3994[1], JW3994, malJ
  • Species
    Escherichia coli (strain K12)
  • Insert Size (bp)
    1191
  • Mutation
    mutations Y283D, G289C and V347C
  • GenBank ID
    948538
  • Promoter T5
  • Tag / Fusion Protein
    • none

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer 5' - GCC CAG AAA GGT GAA ATC - 3'
  • 3′ sequencing primer 5' - AAGGCGAAACAGCGATGA - 3'
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Vector contains T5 promoter, lac operator, laclq repressor gene and Col E1 origin of replication. The original 6xHis tag sequence of pQE-2 has been removed from this construct.

Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAL829 was a gift from Linda Randall (Addgene plasmid # 119697 ; http://n2t.net/addgene:119697 ; RRID:Addgene_119697)
  • For your References section:

    Co-assembly of SecYEG and SecA fully restores the properties of the native translocon. Bariya P, Randall LL. J Bacteriol. 2018 Oct 1. pii: JB.00493-18. doi: 10.1128/JB.00493-18. 10.1128/JB.00493-18 PubMed 30275279