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pBMN mCherry-TAX1BP1(V142S/ΔUBD)
(Plasmid #119690)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 119690 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBMN
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TAX1BP1
  • Alt name
    CALCOCO3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2268
  • Mutation
    V142S & UBD deletion (1-755), See depositor comments below
  • Entrez Gene
    TAX1BP1 (a.k.a. CALCOCO3, T6BP, TXBP151)
  • Tag / Fusion Protein
    • mCherry (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please Note: The Valine to Serine mutation is at position 143 relative to the NCBI Reference Sequence AAH50358.1

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBMN mCherry-TAX1BP1(V142S/ΔUBD) was a gift from Michael Lazarou (Addgene plasmid # 119690 ; http://n2t.net/addgene:119690 ; RRID:Addgene_119690)

  • For your References section:

    LC3/GABARAPs drive ubiquitin-independent recruitment of Optineurin and NDP52 to amplify mitophagy. Padman BS, Nguyen TN, Uoselis L, Skulsuppaisarn M, Nguyen LK, Lazarou M. Nat Commun. 2019 Jan 24;10(1):408. doi: 10.1038/s41467-019-08335-6. 10.1038/s41467-019-08335-6 PubMed 30679426
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