pBMN mCherry-NDP52(V136S/C443K)
(Plasmid
#119686)
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PurposeFor mammalian expression of LIR/UBD double mutant NDP52
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 119686 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBMN
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNDP52
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Alt nameCALCOCO2
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SpeciesH. sapiens (human)
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Insert Size (bp)1335
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MutationV136S/C443K
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Entrez GeneCALCOCO2 (a.k.a. NDP52)
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Tag
/ Fusion Protein
- mCherry (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBMN mCherry-NDP52(V136S/C443K) was a gift from Michael Lazarou (Addgene plasmid # 119686 ; http://n2t.net/addgene:119686 ; RRID:Addgene_119686) -
For your References section:
LC3/GABARAPs drive ubiquitin-independent recruitment of Optineurin and NDP52 to amplify mitophagy. Padman BS, Nguyen TN, Uoselis L, Skulsuppaisarn M, Nguyen LK, Lazarou M. Nat Commun. 2019 Jan 24;10(1):408. doi: 10.1038/s41467-019-08335-6. 10.1038/s41467-019-08335-6 PubMed 30679426