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pBMN mCherry-OPTN(F178A/D474N)
(Plasmid #119684)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 119684 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBMN
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    OPTN
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1727
  • Mutation
    F178A/D474N
  • Entrez Gene
    OPTN (a.k.a. ALS12, FIP2, GLC1E, HIP7, HYPL, NRP, TFIIIA-INTP)
  • Tag / Fusion Protein
    • mCherry (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBMN mCherry-OPTN(F178A/D474N) was a gift from Michael Lazarou (Addgene plasmid # 119684 ; http://n2t.net/addgene:119684 ; RRID:Addgene_119684)

  • For your References section:

    LC3/GABARAPs drive ubiquitin-independent recruitment of Optineurin and NDP52 to amplify mitophagy. Padman BS, Nguyen TN, Uoselis L, Skulsuppaisarn M, Nguyen LK, Lazarou M. Nat Commun. 2019 Jan 24;10(1):408. doi: 10.1038/s41467-019-08335-6. 10.1038/s41467-019-08335-6 PubMed 30679426
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