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Addgene

pFGRNA-AsCpf1-SpCas9
(Plasmid #119674)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 119674 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFGRNA
  • Backbone manufacturer
    Yongsub Kim
  • Backbone size (bp) 3161
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFGRNA-AsCpf1-SpCas9 was a gift from Yongsub Kim (Addgene plasmid # 119674 ; http://n2t.net/addgene:119674 ; RRID:Addgene_119674)

  • For your References section:

    Publisher Correction: Fusion guide RNAs for orthogonal gene manipulation with Cas9 and Cpf1. Kweon J, Jang AH, Kim DE, Yang JW, Yoon M, Rim Shin H, Kim JS, Kim Y. Nat Commun. 2018 Jan 16;9(1):303. doi: 10.1038/s41467-017-02686-8. 10.1038/s41467-017-02686-8 PubMed 29335559
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