pSZBAD1PG
(Plasmid
#11964)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 11964 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBAD
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Backbone manufacturerinvitrogen
- Backbone size w/o insert (bp) 4766
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameSspDnaEC
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Alt nameIntein
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SpeciesSynechocystis sp. PCC6803
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Insert Size (bp)125
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Tag
/ Fusion Protein
- GB1 (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer pBAD (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byFrom Prof. Henry Paulus. Boston Biomedical Research Institute.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSZBAD1PG was a gift from Hideo Iwai (Addgene plasmid # 11964 ; http://n2t.net/addgene:11964 ; RRID:Addgene_11964) -
For your References section:
Intein-based biosynthetic incorporation of unlabeled protein tags into isotopically labeled proteins for NMR studies. Zuger S, Iwai H. Nat Biotechnol. 2005 Jun . 23(6):736-40. 10.1038/nbt1097 PubMed 15908942