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Addgene

pJJDuet30
(Plasmid #11962)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11962 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSFDuet-1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3600
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ssp DnaE-N
  • Alt name
    Intein
  • Insert Size (bp)
    487
  • Tag / Fusion Protein
    • GB1 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer pCW-SEQ-fwd (5'-ACATCGTATAACGTTACTGG-3'​)
  • 3′ sequencing primer T7term (5'-GCTAGTTATTGCTCAGCGG-3'​)
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJJDuet30 was a gift from Hideo Iwai (Addgene plasmid # 11962 ; http://n2t.net/addgene:11962 ; RRID:Addgene_11962)

  • For your References section:

    Intein-based biosynthetic incorporation of unlabeled protein tags into isotopically labeled proteins for NMR studies. Zuger S, Iwai H. Nat Biotechnol. 2005 Jun . 23(6):736-40. 10.1038/nbt1097 PubMed 15908942
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