pBS537 tet-hCMV-GFPcre
(Plasmid #11960)

• Depositing Lab: Brian Sauer
• Publication: Le et al Anal Biochem. 1999 Jun 1. 270(2):334-6.

Backbone

• Vector backbone: na
• Backbone size w/o insert (bp): 4300
• Vector type: Mammalian Expression, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GFP-cre
• Alt name: cre
• Species: bacteriophage P1
• Insert Size (bp): 1850
• Entrez Gene: cre (a.k.a. P1_gp003)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: na

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pBS537 carries a GFPcre fusion gene under the control of a synthetic promoter that can be regulated by a synthetic tet repressor/activator protein. In the presence of doxycycline expression is dramatically turned on to high levels. The GFP moiety carries the S65T mutation for enhanced fluorescence, but is not codon-optimized for mammalian expression.