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Addgene

pGRE GFP
(Plasmid #1196)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1196 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC18 w/ GRE promoter
  • Backbone size w/o insert (bp) 2800
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP w/ stop codon
  • Species
    jellyfish
  • Insert Size (bp)
    720
  • Mutation
    GFP w/ stop codon

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SpeI (destroyed during cloning)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

2 micron yeast plasmid

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGRE GFP was a gift from Susan Lindquist (Addgene plasmid # 1196 ; http://n2t.net/addgene:1196 ; RRID:Addgene_1196)

  • For your References section:

    Support for the prion hypothesis for inheritance of a phenotypic trait in yeast. Patino MM, Liu JJ, Glover JR, Lindquist S. Science 1996 Aug 2;273(5275):622-6. 10.1126/science.273.5275.622 PubMed 8662547
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