pBS595 tet-hCMV-EGFPcre
(Plasmid #11957)

• Depositing Lab: Brian Sauer
• Publication: Le et al Anal Biochem. 1999 Jun 1. 270(2):334-6.

Backbone

• Vector backbone: na
• Backbone size w/o insert (bp): 5200
• Vector type: Mammalian Expression, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EGFP-cre
• Alt name: cre
• Species: bacteriophage P1
• Insert Size (bp): 1800
• Entrez Gene: cre (a.k.a. P1_gp003)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: na

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pBS595 differs from pBS597 in having the GCSF A(n) instead of the MT-I A(n). pBS597 carries the EGFPcre fusion gene under the control of a synthetic promoter that can be regulated by a synthetic tet repressor/activator protein. In the presence of doxycycline expression is dramatically turned on to high levels. The GFP moiety of the fusion gene carries the S65T mutation for enhanced fluorescence and is codon-optimised for higher level expression. Two related constructs are the promoterless vector pBS592 and the vector pBS598 having constitutive high level expression.