CD3 delta YFP
(Plasmid #11951)

• Depositing Lab: Nico Dantuma
• Publication: Menendez-Benito et al Hum Mol Genet. 2005 Oct 1. 14(19):2787-99.

Backbone

• Vector backbone: EGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 3961
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CD3delta-YFP
• Alt name: CD3 delta YFP
• Insert Size (bp): 1265

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: EGFP-N

Resource Information

• A portion of this plasmid was derived from a plasmid made by: CD3 delta amplified from a plasmid from Dr Kikkert, Leiden University Medical Center, Leiden, The Netherlands
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

CD3 delta was PCR amplified from a plasmid, introducing flanking BglII and EcoRI sites, and cloned in Ub-R-YFP digested with BamHI and EcoRI.