YFP-CL1
(Plasmid #11950)

• Depositing Lab: Nico Dantuma
• Publication: Menendez-Benito et al Hum Mol Genet. 2005 Oct 1. 14(19):2787-99.

Backbone

• Vector backbone: EGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 3932
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: YFP-CL1
• Alt name: Ubiquitin
• Alt name: Ub
• Insert Size (bp): 826

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: EGFP-C

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The YFP-CL1 reporter was generated by inserting an oligodimer encoding the 16 amino acid CL1 degradation signal (ACKNWFSSLSHFVIHL) in enhanced GFP-C1 (BD Biosciences Clontech) using the EcoRI and BamH1 restriction sites, then the enhanced GFP open-reading frame was replaced with YFP using the Nhe1 and SspB1 restriction sites.