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Addgene

pcDNA3 HA 14-3-3 sigma
(Plasmid #11946)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11946 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5446
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Stratifin
  • Alt name
    14-3-3 sigma
  • Species
    H. sapiens (human)
  • Entrez Gene
    SFN (a.k.a. YWHAS)
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3 HA 14-3-3 sigma was a gift from Michael Yaffe (Addgene plasmid # 11946 ; http://n2t.net/addgene:11946 ; RRID:Addgene_11946)

  • For your References section:

    A structural basis for 14-3-3sigma functional specificity. Wilker EW, Grant RA, Artim SC, Yaffe MB. J Biol Chem. 2005 May 13. 280(19):18891-8. 10.1074/jbc.M500982200 PubMed 15731107
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