pcDNA3.1/HisC 14-3-3 epsilon K49E
(Plasmid
#11943)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 11943 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepcDNA3.1 His C
-
Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5514
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameYwhae
-
Alt name14-3-3 epsilon
-
SpeciesR. norvegicus (rat)
-
MutationK49E
-
Entrez GeneYwhae (a.k.a. 14-3-3e)
-
Tag
/ Fusion Protein
- His (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pcDNA3.1/HisC 14-3-3 epsilon K49E was a gift from Michael Yaffe (Addgene plasmid # 11943 ; http://n2t.net/addgene:11943 ; RRID:Addgene_11943) -
For your References section:
14-3-3 transits to the nucleus and participates in dynamic nucleocytoplasmic transport. Brunet A, Kanai F, Stehn J, Xu J, Sarbassova D, Frangioni JV, Dalal SN, DeCaprio JA, Greenberg ME, Yaffe MB. J Cell Biol. 2002 Mar 4. 156(5):817-28. 10.1083/jcb.200112059 PubMed 11864996