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Addgene

mRFP-Ub
(Plasmid #11935)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11935 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    EGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ubiquitin
  • Alt name
    Ub
  • Alt name
    ubiquitin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    250
  • Entrez Gene
    RBX1 (a.k.a. BA554C12.1, RNF75, ROC1)
  • Tag / Fusion Protein
    • mRFP (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mRFP-Ub was a gift from Nico Dantuma (Addgene plasmid # 11935 ; http://n2t.net/addgene:11935 ; RRID:Addgene_11935)

  • For your References section:

    DNA damage triggers nucleotide excision repair-dependent monoubiquitylation of histone H2A. Bergink S, Salomons FA, Hoogstraten D, Groothuis TA, de Waard H, Wu J, Yuan L, Citterio E, Houtsmuller AB, Neefjes J, Hoeijmakers JH, Vermeulen W, Dantuma NP. Genes Dev. 2006 May 15. 20(10):1343-52. 10.1101/gad.373706 PubMed 16702407
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