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PHAGE-P CMVt N-HA hE6-AP I (R39H)
(Plasmid #119309)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 119309 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PHAGE
  • Backbone size w/o insert (bp) 7818
  • Total vector size (bp) 10374
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    E6-AP I (R39H)
  • Alt name
    UBE3A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2556
  • Mutation
    G20V
  • Entrez Gene
    UBE3A (a.k.a. ANCR, AS, E6-AP, EPVE6AP, HPVE6A, PIX1)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PHAGE-P CMVt N-HA hE6-AP I (R39H) was a gift from Peter Howley (Addgene plasmid # 119309 ; http://n2t.net/addgene:119309 ; RRID:Addgene_119309)

  • For your References section:

    Angelman syndrome-associated point mutations in the Zn-binding N-terminal (AZUL) domain of UBE3A ubiquitin ligase inhibit binding to the proteasome. Kuhnle S, Martinez-Noel G, Leclere F, Hayes SD, Harper JW, Howley PM. J Biol Chem. 2018 Sep 26. pii: RA118.004653. doi: 10.1074/jbc.RA118.004653. 10.1074/jbc.RA118.004653 PubMed 30257870
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