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Addgene

pAL832
(Plasmid #119305)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 119305 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pET503
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    BL21.19
  • Growth instructions
    Does not require the lambda DE3 lysogen, Change media and shift temperature to 39 degrees with IPTG added to produce the precursor proteins
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    precursor outer membrane protein A, ompA, truncated after amino acid 176 including with mutations T95C and I153C
  • Alt name
    con; ECK0948; tolG; tut
  • Species
    Escherichia coli (strain K12)
  • Insert Size (bp)
    891
  • Mutation
    truncated after amino acid 176, mutations Threonine 95 to Cysteine and Isoleucine 153 to Cysteine
  • GenBank ID
    945571
  • Promoter lac
  • Tag / Fusion Protein
    • none

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer 5' - CAG GGC GTT CAA CTG ACC - 3'
  • 3′ sequencing primer 5' - TCGCCCTGACCGAAACGG - 3'
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Arnold Driessen at University of Groningen in the Neatherlands

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAL832 was a gift from Linda Randall (Addgene plasmid # 119305 ; http://n2t.net/addgene:119305 ; RRID:Addgene_119305)
  • For your References section:

    Co-assembly of SecYEG and SecA fully restores the properties of the native translocon. Bariya P, Randall LL. J Bacteriol. 2018 Oct 1. pii: JB.00493-18. doi: 10.1128/JB.00493-18. 10.1128/JB.00493-18 PubMed 30275279