pCSCIGW-PME1 R369D
(Plasmid
#119296)
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PurposeLentiviral overexpression of full length PPME1 with mutation in the PP2Ac binding motif
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 119296 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCSCIGW
- Backbone size w/o insert (bp) 9755
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePPME1
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SpeciesH. sapiens (human)
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MutationR369D
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Entrez GenePPME1 (a.k.a. PME-1)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site AgeI (not destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCSCIGW-PME1 R369D was a gift from Jukka Westermarck (Addgene plasmid # 119296 ; http://n2t.net/addgene:119296 ; RRID:Addgene_119296)