pcDNA3.1/CIP2A(1-905) R522D V5 His
(Plasmid #119289)

• Purpose: CIP2A R522D V5 overexpression, CIP2A dimer defective mutant
• Depositing Lab: Jukka Westermarck
• Publication: Wang et al EMBO Rep. 2017 Mar;18(3):437-450. doi: 10.15252/embr.201642788. Epub 2017 Feb 7.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 5523
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: KIAA1524
• Species: H. sapiens (human)
• Insert Size (bp): 3000
• Mutation: R522D
• Entrez Gene: CIP2A (a.k.a. KIAA1524, NOCIVA, p90)
• Promoter: T7
• Tag / Fusion Protein:
  • V5 His (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: KpnI (unknown if destroyed)
• 5′ sequencing primer: CMV
• 3′ sequencing primer: BGH

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

522 AGA-> GAT mutation