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Addgene

ID1GFP Donor
(Plasmid #119282)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 119282 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pUC19
  • Backbone size w/o insert (bp) 2700
  • Vector type
    Reporter

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Id1GFP + homology arms
  • Alt name
    idb1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3000
  • Entrez Gene
    Id1 (a.k.a. AI323524, D2Wsu140e, Idb1, bHLHb24)
  • Tag / Fusion Protein
    • eGFP

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer M13_F
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ID1GFP Donor was a gift from Joan Massague (Addgene plasmid # 119282 ; http://n2t.net/addgene:119282 ; RRID:Addgene_119282)
  • For your References section:

    ID1 mediates escape from TGF-beta tumor suppression in pancreatic cancer. Huang YH, Hu J, Chen F, Lecomte N, Basnet H, David CJ, Witkin MD, Allen PJ, Leach SD, Hollmann TJ, Iacobuzio-Donahue CA, Massague J. Cancer Discov. 2019 Oct 3. pii: 2159-8290.CD-19-0529. doi: 10.1158/2159-8290.CD-19-0529. 10.1158/2159-8290.CD-19-0529 PubMed 31582374