ID1GFP Donor
(Plasmid
#119282)
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PurposeMouse ID1-eGFP for HR to create reporter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 119282 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC19
- Backbone size w/o insert (bp) 2700
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Vector typeReporter
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameId1GFP + homology arms
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Alt nameidb1
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SpeciesM. musculus (mouse)
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Insert Size (bp)3000
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Entrez GeneId1 (a.k.a. AI323524, D2Wsu140e, Idb1, bHLHb24)
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Tag
/ Fusion Protein
- eGFP
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site XbaI (unknown if destroyed)
- 5′ sequencing primer M13_F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ID1GFP Donor was a gift from Joan Massague (Addgene plasmid # 119282 ; http://n2t.net/addgene:119282 ; RRID:Addgene_119282) -
For your References section:
ID1 mediates escape from TGF-beta tumor suppression in pancreatic cancer. Huang YH, Hu J, Chen F, Lecomte N, Basnet H, David CJ, Witkin MD, Allen PJ, Leach SD, Hollmann TJ, Iacobuzio-Donahue CA, Massague J. Cancer Discov. 2019 Oct 3. pii: 2159-8290.CD-19-0529. doi: 10.1158/2159-8290.CD-19-0529. 10.1158/2159-8290.CD-19-0529 PubMed 31582374