ID1-pLVXT
(Plasmid
#119280)
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PurposeDoxycycline-inducible mouse ID1 from 806 cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 119280 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLVX-tight-Puro
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 7800
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameId1
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SpeciesM. musculus (mouse)
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Insert Size (bp)447
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GenBank ID
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Entrez GeneId1 (a.k.a. AI323524, D2Wsu140e, Idb1, bHLHb24)
- Promoter TRE
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer pLVXT (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ID1-pLVXT was a gift from Joan Massague (Addgene plasmid # 119280 ; http://n2t.net/addgene:119280 ; RRID:Addgene_119280) -
For your References section:
ID1 mediates escape from TGF-beta tumor suppression in pancreatic cancer. Huang YH, Hu J, Chen F, Lecomte N, Basnet H, David CJ, Witkin MD, Allen PJ, Leach SD, Hollmann TJ, Iacobuzio-Donahue CA, Massague J. Cancer Discov. 2019 Oct 3. pii: 2159-8290.CD-19-0529. doi: 10.1158/2159-8290.CD-19-0529. 10.1158/2159-8290.CD-19-0529 PubMed 31582374
Map uploaded by the depositor.
