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pCD4-TagRFP-T (RHPMP151)
(Plasmid #119238)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 119238 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1+
  • Backbone manufacturer
    ThermoFisher
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CD4
  • Alt name
    cluster of differentiation 4
  • Species
    H. sapiens (human)
  • GenBank ID
    BC025782.1
  • Entrez Gene
    CD4 (a.k.a. CD4mut, IMD79, Leu-3, OKT4D, T4)
  • Promoter CMV
  • Tag / Fusion Protein
    • tagRFP-T (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CAACGGGACTTTCCAAAA
  • 3′ sequencing primer CTAGAAGGCACAGTCGAG
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCD4-TagRFP-T (RHPMP151) was a gift from Ricardo Henriques (Addgene plasmid # 119238 ; http://n2t.net/addgene:119238 ; RRID:Addgene_119238)

  • For your References section:

    Fix Your Membrane Receptor Imaging: Actin Cytoskeleton and CD4 Membrane Organization Disruption by Chemical Fixation. Pereira PM, Albrecht D, Culley S, Jacobs C, Marsh M, Mercer J, Henriques R. Front Immunol. 2019 Apr 5;10:675. doi: 10.3389/fimmu.2019.00675. eCollection 2019. 10.3389/fimmu.2019.00675 PubMed 31024536
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